Table 2. Oligonucleotide primers designed and used for wild-type or hybrid colicin cloning.
| Primer name | Sequence 5′-3′ | Function | Source |
| Nt-ColE7 | GGAATTCCATATGAGCGGTGGaGATGGAC | ColE7 sense cpa | Twb |
| Ct-ImmE7 | TCGCTCG GATCC TCAGTGGTGGTGGTGGTGGTGGCCCTGTTTAAATCCTGGCTTAC | ColE7 antisense cp | Tw |
| Nt-ColE2 | GGAATTCCATATGAGCGGTGGcGATGGAC | ColE2 sense cp | Tw |
| Ct-ImmE2 | TCGCTCGGATCC TCAGTGGTGGTGGTGGTGGTGGCCCTGTTTAAATCCTGACTTACCGTTAG | ColE2 antisense cp | Tw |
| Nt-ColE3 | GGAATTCCATATGAGCGGTGGCGATGGACGCGGCCATAACACG | ColE3 sense cp | Tw |
| Ct-ImmE3 | TCGCGGATCCATTAGTGGTGGTGGTGGTGGTGCCAATCACCATCACGATAATCAAACGAAAC | ColE3 antisense cp | Tw |
| ColE2-G456 | CTTCCCTGGCTTATTCCGTTTACTCTCC | ColE2 antisense p G456 | Tw |
| ColE3-R454 | TTTTCTGGGCTTATTCTTTTCATCG | ColE3 antisense p R454 | Tw |
| ColE3-ColE2 | GGAGTGCTGAAAATAATTTAAACGATGAAAAGAATAAGCCCAGAAAAGCGACAGGTAAAGGTAAACCAGTTG | Jonction sense pColE3-R440-R454/ColE2- K457-V465 | Tw |
| ColE2-ColE3 | CTAAGGATAAATTAGATAAGGAGAGTAAACGGAATAAGCCAGGGAAGGGTTTTAAAGATTACGGGCATGATTATCATCC | Jonction sense pColE2-A442-G456/ColE3-K455-H465 | Tw |
a
Cloning primer (NdeI or BamH1 restriction sites are in bold italic; start or stop codons are double underlined; C-terminal 6His-tag is underlined).
b
This work.