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PreB697/EU3 cells were infected with lentiviral particles encoding a shRNA targeting BIM in combination with either eGFP- or dtTOMATO- fluorescence reporters. (A) PreB697/EU3 cells were sorted either for eGFP (left panel, R1), dtTOMATO- (middle panel, R2) or eGFP- and dtTOMATO- (right panel, R3) expression. (B) Protein lysates of sorted cells were analysed for BIM and α-tubulin expression by immunoblotting.
