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. 2014 May 19;9(5):e97764. doi: 10.1371/journal.pone.0097764

Figure 4. Conditional silencing using the one-vector GLTR-X system.

Figure 4

U2OS cells were infected with pGLTR-X-GFP-CDC27. Infected cells were cultured in the presence or absence of 1 µg/ml Dox for 3 days and analysed by microscopy. Induced cells reveal a mitotic arrest phenotype (A). For time-course experiments (B), cells were treated with 1 µg/ml Dox and samples taken at the indicated time points for immunoblotting for CDC27 levels as well as GFP as loading control. For dose-response analysis (C), cells were treated with increasing doses of Dox for 3 days and total cell extracts analysed for CDC27 and GFP expression.