Figure 6. Conditional gene silencing in primary human HUVEC cells.

pGLTR-X-GFP-CDC27 was used to infect primary HUVECs. Infected cells were induced 5 days after infection for 3 days with 1 µg/ml Dox and analysed by phase contrast and fluorescence microscopy. Mitotic cells are labelled by arrows (A). For dose-response analysis (B), cells were treated with increasing doses of Dox for 3 days and total cell extracts analysed for CDC27 and GAPDH expression.