Table 2.
Primer pairs used for PCR amplification and Sanger sequencing
| Locus | Fw primer | Rv primer | Annealing temp. (°C) | Size 1 (bp) | Seq. composition (bp) 2 | Alleles 3 | H 4 |
|---|---|---|---|---|---|---|---|
|
TEF |
EF1: ATGGGTAAGGARGACAAGAC |
EF2: GGARGTACCAGTSATCATGTT |
53 |
667 |
256 intronic; 411 exonic |
22 |
0.89 |
| rDNA |
ITS5: GGAAGTAAAAGTCGTAACAAGG |
NL4: GGTCCGTGTTTCAAGACGG |
53 |
1029 |
705 coding; 324 ITS |
6 |
0.35 |
|
RPB2 5-7 |
5f2: GGGGWGAYCAGAAGAAGGC |
7cr: CCCATRGCTTGYTTRCCCAT |
55 |
863 |
Exonic |
11 |
0.51 |
|
RPB2 7-11 |
7cf: ATGGGYAARCAAGCYATGGG |
11ar: GCRTGGATCTTRTCRTCSACC |
55 |
881 |
Exonic |
- |
- |
| 3968 |
3968fw: TGTTGGTTCGAGATGGTTGA |
3968rv: GAGAAGGGCAACTGGGAGA |
53 |
770 |
Intergenic |
12 |
0.68 |
| 3972 |
3972fw: TCTGATGCAGACTAGCACTCG |
3972rv: ATCGGACGAAACAGAGCAGG |
53 |
831 |
Intergenic |
13 |
0.45 |
| 4081 |
4081fw: TGACRAGGATGAATGAGCGA |
4081rv: TGACCAGCCTCCAAGSG |
56 |
642 |
Intergenic |
18 |
0.8 |
| 6512 |
6512fw: GGAGGACCAGGAGGAATAGG |
6512rv: CAAAGCAGATCGACTGAGGA |
53 |
644 |
Intergenic |
12 |
0.52 |
| 5439 |
5439fw: AATGGGAATACGAGCGTCAG |
5439rv: AGGGGCTGCTGTTAGTGAGA |
53 |
779 |
Intergenic |
10 |
0.48 |
| 5437 |
5437fw: AACAAGACAAGGCAGCAGGT |
5437rv: TCCAGAGGAACGACGAGGC |
56 |
544 |
213 exonic, 331 intergenic |
23 |
0.88 |
|
MAT1-1 |
MAT1-S-1 F: ATGGCTTTCCGCAGTAAGGA |
MAT1-S-1R: CATGATAGGGCAGCAAAGAG |
53 |
~200 |
N. A. |
N. A. |
N. A. |
| MAT1-2 | MAT2-S-1 F: GGGAATCTGAGAAAGATACGTAC | MAT2-S-1R: CGGTACTGGTAGTCGGGAT | 53 | ~800 | N. A. | N. A. | N. A. |
1. Number of characters in the aligned dataset. For different isolates, the exact length of any region may be variable due to indels and microsatellite repeats 2. ITS = internal transcribed spacers 1 and 2. Locus 5437 contains coding regions of a predicted hypothetical protein of unknown function. 3. Number of alleles observed in F. keratoplasticum 4. Average genetic diversity of each of the 9 loci calculated using LIAN accessed at http://pubmlst.org.