Table 1. qRT-PCR primer sets used in this study.
Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Amplicon size (bp) | Efficiency (%) | Correlation coefficient (R2) |
16S rRNA | ACACATGCAAGTCGAACGAG | CGTCCGTTTCCAGACGTTAT | 100 | 86 | 0.996 |
gapB | CTTCGTGCTGAACAAGCTGA | AGGTCCTTGTGGTTGGTGTC | 102 | 109.1 | 0.997 |
glyA | GGAGATCGCCAAGAAGATCA | GCTCTTGGCGTAGGTCTTGA | 133 | 104 | 0.998 |
gyrA | TCACCGACGAAGAGTTGATG | CTCTTCGATCTCGGTCTTGG | 143 | 98.4 | 0.999 |
proC | CAAAACCATCGCCTCCTTC | AATCGCACAGCGACTTCTG | 149 | 106.6 | 0.988 |
pykA | GACCTTTACCGCACGATGAT | TGGTGGTGTAGGTGACGATG | 130 | 101 | 0.996 |
recA | GTCGAACTGCCTGGTGATCT | GACGGAGGCGTAGAACTTCA | 112 | 95.2 | 0.997 |
recF | GCTGTTCGACGAGATCCTG | CTCGATGTGGAAGCGTTTG | 106 | 109.1 | 0.996 |
rpoD | CGTCACCTATGACGAGCTGA | CTCTTCCGATTCGACGATGT | 118 | 102.2 | 0.998 |
tpiA | GGTCCTCTACGGCGGTTC | AGAAATCGTCCGCCTTCAG | 110 | 96.8 | 0.999 |