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. 2014 May 19;9(5):e98162. doi: 10.1371/journal.pone.0098162

Table 1. qRT-PCR primer sets used in this study.

Gene Forward Primer (5′-3′) Reverse Primer (5′-3′) Amplicon size (bp) Efficiency (%) Correlation coefficient (R2)
16S rRNA ACACATGCAAGTCGAACGAG CGTCCGTTTCCAGACGTTAT 100 86 0.996
gapB CTTCGTGCTGAACAAGCTGA AGGTCCTTGTGGTTGGTGTC 102 109.1 0.997
glyA GGAGATCGCCAAGAAGATCA GCTCTTGGCGTAGGTCTTGA 133 104 0.998
gyrA TCACCGACGAAGAGTTGATG CTCTTCGATCTCGGTCTTGG 143 98.4 0.999
proC CAAAACCATCGCCTCCTTC AATCGCACAGCGACTTCTG 149 106.6 0.988
pykA GACCTTTACCGCACGATGAT TGGTGGTGTAGGTGACGATG 130 101 0.996
recA GTCGAACTGCCTGGTGATCT GACGGAGGCGTAGAACTTCA 112 95.2 0.997
recF GCTGTTCGACGAGATCCTG CTCGATGTGGAAGCGTTTG 106 109.1 0.996
rpoD CGTCACCTATGACGAGCTGA CTCTTCCGATTCGACGATGT 118 102.2 0.998
tpiA GGTCCTCTACGGCGGTTC AGAAATCGTCCGCCTTCAG 110 96.8 0.999