FIG. 4.

GSH and GSSG levels in lung tissues after exposure to LPS and hyperoxia for 24 or 72 h. Adult mice received intratracheal saline or LPS at 12 h before exposure to RA or hyperoxia (>95% O₂). (A) At 24 h, GSH and GSSG levels were determined and GSH/GSSG ratios were calculated as described in the Materials and Methods section. Data (mean±SD) were analyzed by two-way ANOVA with Tukey's multiple-comparisons test post hoc (n=3–5). *p<0.002 versus saline/RA, ^%p<0.0006 versus LPS/RA. (B) At 48 h, GSH and GSSG levels were determined and GSH/GSSG ratios were calculated as described in the Materials and Methods section. Data (mean±SD) were analyzed by two-way ANOVA with Tukey's multiple-comparisons test post hoc (n=3–5). *p<0.04 versus saline/RA, ^%p<0.02 versus LPS/RA. GSH, glutathione; GSSG, GSH disulfide.