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. 2014 May 19;9(5):e97994. doi: 10.1371/journal.pone.0097994

Figure 4. Western blotting analysis of proteasomal degradation and stability of Ile60Asn parafibromin.

Figure 4

A. Western blotting for parafibromin (with anti-CDC73 antibody) in HeLa cells in absence (−) or in presence (+) of the proteasome inhibitor MG132. B. Western blotting for parafibromin in HeLa cells after treatment with CHX (0 = no treatment) and harvesting at the indicated time points. C. Graphical representation of the stability of wild-type parafibromin (WT-CDC73) and Ile60Asn mutant at different time points after treatment with CHX.