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. 2014 May 19;9(5):e97994. doi: 10.1371/journal.pone.0097994

Figure 7. Flow cytometry analysis of cell cycle in transfected HEK293A cells.

Figure 7

Cell cycle analysis with flow cytometry. HEK293A cells were transfected with the control vector (tGFP) or fusion proteins of tGFP with wild-type (WT-CDC73) or Ile60Asn mutant parafibromin (Ile60Asn), and the analysis was performed 48 hours after transfection. A. Flow cytometric analysis plotting cell number vs. intensity of propidium iodide (PI) signal from a representative experiment. B. Histograms showing the percentages of cells in the different phases of cell cycle. Values shown represent mean and standard deviations of pooled results from 4 independent experiments; *, P<0.05 compared to control cells; **, P<0.05 compared to wild-type parafibromin transfected cells; ***P<0.05 compared to both wild-type parafibromin-transfected cells and control cells.