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. 2014 May 7;5(6):457–468. doi: 10.1007/s13238-014-0055-y

Figure 1.

Figure 1

Effect of PI4KIIα knockdown on both HER-2 activity and HER-2/EGFR interaction. (A) Specific regulation of HER-2 by PI4KIIα. MCF-7 cells were transfected with either control siRNA, PI4KIIα siRNA or PI4KIIβ siRNA, and treated with 100 ng/mL EGF for 10 min. The autophosphorylation level of HER-2 at Tyr1248 and the protein expression levels as indicated were measured by Western blot. (B) The rescue effect of PI4P on HER-2 activity following PI4KIIα knockdown. (C) Effect of PI4KIIα knockdown on the interaction of HER-2 with EGFR. Lysates of treated MCF-7 cells (prior to addition of EGF for 10 min) were subjected to immunoprecipitation with anti-EGFR antibody or rabbit IgG respectively. The immunoprecipitates were immunoblotted with anti-HER-2 antibody and EGFR antibody. Loading controls are shown in the lower panels (Input). All results presented above represent data from three independent experiments