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. 2014 May 19;9(5):e97942. doi: 10.1371/journal.pone.0097942

Figure 2. Src kinase represses osteocalcin in static and OFSS conditions.

Figure 2

(A) Treating MC3T3 osteoblasts and MLO-Y4 osteocytes with Src Inhibitor 1 (SI1) (10 µM) for 1 hour inhibits Src kinase activity. Western blot analysis of phosphorylated-Akt (Ser308), Total Akt, and γ-tubulin under static and OFSS conditions (1 hour). (B) One hour of SI1 treatment significantly increases the expression of osteocalcin in MC3T3 osteoblasts and MLO-Y4 osteocytes (*p<0.05). (C) OFSS (1 hour) further enhanced the expression of osteocalcin in both MC3T3 osteoblasts and MLO-Y4 osteocytes compared to static controls treated with carrier (DMSO) (p<0.05). *Represents a statically significant increase compared to wild-type static control (p<0.05). #Represents a statically significant difference between treatment groups (#p<0.05) Error bars represent standard error. An n≥3 were used in all experiments and each experiment was repeated at least three times.