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. 2014 May 19;9(5):e97890. doi: 10.1371/journal.pone.0097890

Figure 2. Construction of the stable transfection plasmid pEf-msa-1-Bm86ep-gfp-bsd.

Figure 2

A. Schematic description and design of the msa-1-Bm86ep synthetic chimera gene using the sequences encoding for the Bm86 epitopes indicated by the red bar replacing the hyper-variable coding region (HVR) of msa-1, indicated in orange. The white bar represents a 3′ region of msa-1 used to control stop of transcription B. The schematic components of stable transfection plasmid pEf-msa-1-Bm86ep-gfp-bsd are shown. The dark and light blue bars represent the 5′ and 3′ regions of the ef-1α ORF, the yellow bars represent the 1.4 kbp ef-1α IG region, the composite orange and red bar represent the msa-1-Bm86ep chimera with the 3′ msa-1 as shown under the GeneBank accession number for the compiled sequence (KJ598130), the light and dark green bars represent gfp and bsd respectively, and the grey bar represents a 3′ region of rap-1 used to control stop of transcription. C. The ef-1α locus of the B. bovis genome is represented and the arrows indicate where the homologous recombination event of the transfection plasmid is designed to occur.