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. 2014 May 19;9(5):e97890. doi: 10.1371/journal.pone.0097890

Figure 3. Integration of transfected sequences into the genomic ef-1α locus of parasite line Tf-Bm86ep-gfp-bsd.

Figure 3

A. Schematic of the proposed integration site of pEf-msa-1-Bm86ep-gfp-bsd into the ef-1α ORF of the B. bovis ef-1α locus. Large brackets with GenBank accession numbers KJ598132 and KJ598131 represent the regions amplified and sequenced to demonstrate integration of transfected genes into B. bovis ef-1α B ORF locus. Smaller brackets represent the regions of hybridization for southern blot probes A-C. Relative positions of BglII restriction sites are shown at each end of the schematic. B. Shows the results of southern hybridization analysis using dig-labeled probes against ef-1α (Probe A), Bm86ep (Probe B), and msa-1 (Probe C) specific dig-labeled specific probes. Each DNA sample was analyzed using undigested (-) or digested (+) BglII restriction enzyme. Sample order is as follows: 1) dig-labeled DNA ladder, 2) Mo7 wild type parasites, 3) plasmid pBS, 4) Tf-Bm86ep-gfp-bsd, 5) pEf-msa-1-Bm86ep-gfp-bsd.