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. 2014 May 19;9(5):e97890. doi: 10.1371/journal.pone.0097890

Figure 5. Bm86 epitopes are expressed on the surface of Tf-Bm86ep-gfp-bsd extraerythrocytic merozoites.

Figure 5

A–D represent permeabilized extraerythrocytic merozoites stained with DAPI (A), incubated with Bm86ep antibody labeled with Alexa Flour 647 (B), and GFP antibody labeled with Alexa Flour 488 (C). A merged image of panels B and C is shown in panel D. E–H represents identical staining procedures applied to non-permeabilized cells. I–L represent non-permeabilized extraerythrocytic merozoites stained with DAPI (I), and incubated with Bm86ep antibody labeled with Alexa Flour 647 (J), and the MSA-1 mAb Babb35 labeled with Alexa Flour 488 (K). A merged image of panels J and K is shown in panel L. A two micron size bar is included on the bottom right panel.