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. 2014 May 19;9(5):e98315. doi: 10.1371/journal.pone.0098315

Figure 3. Fluorescence microscopy showing trogocytosis induced with 22*-(20)-(20).

Figure 3

Purified monocytes labeled with PKH26-Red fluorescence were mixed 2∶1 with Daudi cells labeled with PKH67-Green fluorescence, and treated with 22*-(20)-(20) (A–C) or labetuzumab (D) at 10 µg/mL. Fluorescent images were captured after 30 min at room temperature with an Olympus BX66 microscope (Shinjuko, Tokyo, Japan) equipped with a Mercury-100W laser (Chiu Technical Corp., Kings Park, NY), using an Olympus 40X/0.75 air objective lens and a Kodak DC290 Camera (Rochester, New York) set at 115X zoom. A WB filter was used to allow simultaneous fluorescence of both red and green fluorochromes. Images were captured and processed using Adobe Photoshop CS3 v.10 software with a Kodak Microscopy Documentation System 290 plug-in application. Bars: 10 µm.