Figure 3. qRT-PCR validation of selected genes in response to ARHGEF3 and RHOA gene knockdown in osteoclast-like cells.

(A) CCL5 mRNA expression in osteoclast-like cells. (B) OSCAR mRNA expression in osteoclast-like cells. (C) ARHGDIA mRNA expression in osteoclast-like cells. (D) ACTA2 mRNA expression in osteoclast-like cells. Data displayed as mean 2^−ΔC _T ± SEM from three biological replicates. *P<0.05, **P<0.01, † determined as significant by ANOVA.