Table 2. Genes significantly influenced in microarray analysis of ARHGEF3 and RHOA gene knockdown in osteoclast-like cells from donor 1.

Knockdown	Gene	Gene product	Meanknockdown*FU	Meancontrol*FU	Expressionratio	P
ARHGEF3	CCL5	Chemokine ligand 5	4402	588	7.48	<0.001
	HLA-C	Major histocompatibility complex,class I, C	235	87	2.71	<0.001
	SNCA	Synuclein, alpha	129	260	0.49	<0.001
	TNF	Tumour necrosisfactor alpha	420	156	2.69	<0.001
	OSCAR	Osteoclast associatedIg-like receptor	1950	1244	1.57	0.01
	CD44	CD44 molecule	2279	3360	0.68	0.03
	BIRC3	Baculoviral IAP repeat-containing 3	496	321	1.55	0.04
	ITGB7	Integrin, beta 7	588	106	5.54	<0.001
	ITGAE	Integrin, alpha E	256	483	0.53	<0.001
	ITGAL	Integrin, alpha L	145	79	1.85	0.003
	ITGA3	Integrin, alpha 3	107	221	0.48	0.003
	ITGAM	Integrin, alpha M	1033	1699	0.61	0.004
RHOA	TNF	Tumour necrosisfactor alpha	345	156	2.21	<0.001
	THBS2	Thrombospondin 2	89	43	2.05	<0.001
	CCL5	Chemokine ligand 5	891	588	1.52	0.006
	ITGB7	Integrin, beta 7	246	106	2.32	<0.001
	ARHGDIA	Rho GDP dissociationinhibitor alpha	579	998	0.58	<0.001
	IGF1	Insulin-like growth factor 1	121	42	2.86	<0.001
	ACTA2	Alpha 2 actin, smooth muscle	545	965	0.56	0.001
	MYL9	Myosin, light chain 9, regulatory	22	49	0.46	0.04
	ITGAE	Integrin, alpha E	342	483	0.71	0.04

Expression ratios are given as expression of the gene in the knockdown cultures relative to the negative control cultures. P adjusted for multiple testing.

*FU, fluorescence units.