Skip to main content
. 2014 May 19;9(5):e97909. doi: 10.1371/journal.pone.0097909

Figure 3. Estrogen increases levels of Sema4D mRNA, but not Plexin-B1 mRNA in mouse vagina.

Figure 3

(A) Sema4D mRNA levels were significantly lower in vaginal tissues from OVX WT mice than in vaginal tissues from sham-operated mice. Based on comparisons between OVX-E2 and OVX-oil mice, OVX-E2 treatment induced a significant increase of Sema4D mRNA levels in WT vaginal tissues. The Sema4D mRNA variant transcribed in Sema4D−/− mice, but not translated into Sema4D protein exhibits an expression pattern similar to that of wild-type Sema4D mRNA. Each value represents the mean ± SEM of 5 mice. *P<0.05, ANOVA. (B) Plexin-B1 mRNA levels in vaginal tissues from any Sema4D−/− mice group were significantly higher than those in vaginal tissues from any WT mice group. In WT or Sema4D−/− mice, any treatment does not induce any significant alteration of Plexin-B1 mRNA levels in vaginal tissues. Each value represents the mean ± SEM of 5 mice. *P<0.05, ANOVA.