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. 2014 May 19;9(5):e97909. doi: 10.1371/journal.pone.0097909

Figure 6. Sema4D contributes to the estrogen-dependent dephosphorylation of Akt and ERK during mouse vaginal opening.

Figure 6

Western blot shows that ovariectomy (OVX) and ovariectomy plus oil (OVX-oil) both increase the phosphorylation level of Akt and ERK1/2 in vaginal tissue from 5-week-old WT (Sema4D+/+) mice. β-estradiol (E2) supplementation after ovariectomy (OVX-E2) induces significant dephosphorylation of Akt and ERK1/2 in vaginal tissue from 5-week-old WT mice. OVX and OVX-oil do not significantly increase phosphorylation of Akt and ERK1/2 in vaginal tissue from 5-week-old Sema4D−/− mice relative to that in sham-operated mice (Sham). OVX-E2 induces weak but significant dephosphorylation of Akt and ERK in vaginal tissue from 5-week-old Sema4D−/− mice. Dephosphorylation levels in the Sema4D−/− vagina is significantly lower than that in the WT vagina. Each data point represents the mean ± SEM of 3 to 6 mice. *P<0.05, ANOVA.