Table 1.
Detection characteristics of MMSR assay in comparison to “wet” assay
|
MMSR assay |
Wet assay |
Parasite/μL | |||
|---|---|---|---|---|---|
| C T Mean | C T SD | C T Mean | C T SD | ||
|
PLU assay |
18.55 |
0.013 |
19.78 |
0.191 |
78240 |
|
FAL assay |
20.07 |
0.031 |
20.85 |
0.055 |
|
|
RNaseP assay |
24.13 |
0.083 |
30.09 |
0.081 |
|
|
PLU assay |
20.73 |
0.092 |
22.17 |
0.227 |
15648 |
|
FAL assay |
22.23 |
0.054 |
23.62 |
0.338 |
|
|
RNaseP assay |
24.52 |
0.185 |
29.57 |
0.082 |
|
|
PLU assay |
23.58 |
0.029 |
24.89 |
0.616 |
3129 |
|
FAL assay |
24.93 |
0.001 |
26.06 |
0.029 |
|
| RNaseP assay | 25.37 | 0.055 | 29.43 | 0.492 | |
NIBSC standard DNA was five-fold serially diluted times. The performance of MMSR assay was compared to the “wet” assay. The performance of the MMSR assay was comparable to that of the “wet” assay for the three assay targets tested.