Table 1.
Sequences of primers
| Cytokine | Sense | Anti-sense |
|---|---|---|
| IL-32α |
5′GCTGGAGGACGACTTCAAAGA3′ |
5′GGGCTCCGTAGGACTTGTCA3′ |
| IL-32β |
5′CAGTGGAGCTGGGTCATCTCA3′ |
5′GGGCCTTCAGCTTCTTCATGTCATCA3′ |
| IL-32γ |
5′AGGCCCGAATGGTAATGCT3′ |
5′CCACAGTGTCCTCAGTGTCACA3′ |
| IL-32δ |
5′TCTGTCTCTCTCGGGTCCTCTCT3′ |
5′TGTCTCCAGGTAGCCCTCTTTG3′ |
| IL-10 |
5′GGTTCGCAAGCCTTGTCTGA 3′ |
5′TCCCCCAGGGAGTTCACAT 3′ |
| TNF |
5′AAGCCTGTAGCCCATGTTGT 3′ |
5′CAGATAGATGGGCTCATACC 3′ |
| GAPDH | 5′CTCAAGATTGTCAGCAATGC 3′ | 5′ CAGGATGCCCTTTAGTGGGC 3′ |
Sequences of primers used to detect cytokine mRNA by using qPCR in leishmaniasis lesions and control tissues.