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The 2,125 endogenous metabolite features detected in 3 × 105 murine macrophage extracts after data reduction. The radius of the data markers (filled circle) reflected the relative abundances of the metabolite features over a dynamic range of 5 × 104. Small metabolites (90–400 m/z) in orange, PGs in green, PEs in red, PCs in purple and lyso-PCs in blue were highlighted. The HILIC gradient was labelled with a black line with reference to percentage acetonitrile
