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. 2014 May 13;5:126. doi: 10.3389/fgene.2014.00126

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Copyright © 2014 Doherty and Couldrey.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CpG coverage generated from RRBS when smaller amounts of data are available for analysis. RRBS data were randomly sampled from the original fastq file to create smaller data sets, (A) Seqmonk screen shot illustrating CpG site coverage across selected genes on chromosome 1, bar height represents a count of CpG coverage, illustrating inclusions of increasing number of genes analyzed as the number of sequences included increases; (B) The number of CpGs covered in these sequentially smaller datasets was identified, in addition to the number of CpGs with at least 10x coverage.