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. 2014 May 12;5:152. doi: 10.3389/fmicb.2014.00152

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Copyright © 2014 Nonaka, Maruyama, Onishi, Kobayashi, Ogura, Hayashi, Suzuki and Masuda.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of plasmid(s) in the donors and transconjugants using Southern hybridization following to pulsed field gel electrophoresis with the tet(M) probe (A), traI-pAQU1 probe (B), and rep-pAQU1 probe (C). Lane: M, DNA size standard (lambda ladder); 1, 04Ya001; 2, 04Ya016; 3, 04Ya090; 4, 04Ya108; 5, 04Ya265; 6, 04Ya311; 7, TJ001W1; 8, TJ016W1; 9, TJ090W1; 10, TJ108W1; 11, TJ265W1; 12, TJ311W2 and 13, W3110. Approximately, 20 ng of DNA was loaded in each lane. Arrows indicate the positions of the bands.