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Detection of a plasmid or ICE(s) in the donors and transconjugants using Southern hybridization following to pulsed field gel electrophoresis with the tet(M) probe (A), traI-ICEVchMex probe (B), and rep-pAQU1 probe. (C) Lane: M, DNA size standard (lambda ladder); 1, 04Ya007; 2, TJ007W1; 3, W3110. Approximately, 20 ng of DNA was loaded in each lane. Arrows indicate the positions of the bands.
