Table 1.
PCR primers used in this study.
| Gene (s) | MGE (s) | Primer name | Primer sequence (5′ -> 3′) | Annealing temperature (°C) | Size (bp) | References |
|---|---|---|---|---|---|---|
| traI | pAQU1 (GenBank accession number AB571865) | MOBH1_traI062-1F MOBH1_traI062-2R | CAYYTRYTRCCAGCWTCYGA SRCGYCWSGCTSATTGAC | 57 | 645 | This study |
| IncA/C plasmids* | ||||||
| SXT/R391 family of ICEs** | ||||||
| tet(B) | pAQU1 (AB571865) | TetB-FW | TACGTGAATTTATTGCTTCGG | 65 | 226 | This study |
| TetB-RV | ATACAGCATCCAAAGCGCAC | |||||
| tet(M) | tet(M)-1 | GTTAAATAGTGTTCTTGGAG | 55 | 676 | Nonaka et al., 2007 | |
| tet(M)-2 | CTAAGATATGGCTCTAACAA | |||||
| blaCARB-9-like | cds084-1f | ATGAGTACATTCAAGACATT | 55 | 672 | This study | |
| cds084-672r | TTATTTGACAGCATACTCTT | |||||
| mph(A)-like | cds165-1f | ATGAAAAATAGAGATATTCA | 55 | 885 | This study | |
| cds165-885r | CTACTCAACACCTAACTGTA | |||||
| mef(A)-like | cds166-1f | ATGGAAAACCGTAAATGGTT | 55 | 1,224 | This study | |
| cds166-1124r | TTAAATATTTTTGATTTTAC | |||||
| sul2 | sul2-F | CTCAATGATATTCGCGGTTTYCC | 58 | 245 | Heuer and Smalla, 2007 | |
| sul2-R | AAAAACCCCATGCCGGGRTC | |||||
| floR | FloR283f | GGAGCAGCTTGGTCTTCAAC | 55 | 226 | This study | |
| FloR519r | AATGAATATCGCCTGCCATC | |||||
| repA | CDS001(AB571865)-446f | GGAAGAAGCATCCAGAGCAC | 55 | 236 | This study | |
| CDS001(AB571865)-646r | AGCCAGAGACGCTGTTGATT | |||||
| traA2 | traA89f | AAGCTCACGCTGGTACTGGT | 67 | 177 | This study | |
| traA246r | CATTGCGAATGCCATGATAC | |||||
| traB | traB343f | CGCGATGTTAAGGAGCTTTC | 55 | 268 | This study | |
| traB591r | CTTACCCGTTGGTTTGCTGT | |||||
| traC | traC1742f | CCGCGTATACCGCTTATGTT | 55 | 388 | This study | |
| traC2110r | CAGCAGGATCGTCAAAGTGA | |||||
| traF | traF1f | GTGGGATAACCCAACCATTG | 55 | 298 | This study | |
| traF1r | GGAGCCTGTATTTCGCACAT | |||||
| traN | traN535f | CCAGGCGATGTGGTTAGTTT | 67 | 1,085 | This study | |
| traN1600r | CTTTGAGAGCCTGAATTGCC | |||||
| traI | traI1976f | ATGTTGATGTGCGTGGTCAT | 55 | 270 | This study | |
| traI2225r | CTTCCGGTTTGGATGAACTG | |||||
| traL | TraL70f | CTGGCTCCTATGCTTCTTGG | 55 | 220 | This study | |
| TraL270r | CCTCCGGATAAATGGGTTTT | |||||
| Int | ICEVchMex (DQ180350) | int575f | TCACAGCAAAGAAAGTCGGC | 57 | 229 | This study |
| int803r | CGGATCTCTATGGGCACTGT | |||||
| traF | traF517f | CGTTCGGATTGCCCTTACTG | 57 | 192 | This study | |
| traF708r | AACTAAGAACACGGCAGGGA | |||||
| traN | traN1087f | ATTCAATGCACGGATCAGCC | 57 | 202 | This study | |
| traN1288r | CACAGGAAGGATTCGCTTCG |