Table 2.
PCR primers for detection of the conserved region between pAQU1 and pAQU2 (pAQU backbone).
| Region number | Primer name | Primer sequence (5′ -> 3′) | Product size (bp) | Relevant genes contained in the amplicon |
|---|---|---|---|---|
| 1 | AB571865-1 | ATGACAACAACACTCCACAATGAGG | 12,606 | repA |
| 170-16f | GCCGCAACGATGACAAACTTAACGA | |||
| 2 | AB571865-30966 | ACAGTTCACCTAAACATTGATG | 3,785 | parA, parB |
| AB571865-34750 | ACATACAACCAGAAACAGAACC | |||
| 3 | 170-11r | GCTTTGGCGTGTTCTCGGTTATGAT | 9,017 | traI, traD |
| 170-11f | CGCATGGAACACTACACCGCTATAT | |||
| 4 | 170-10r | GGTTGCTTCACTGCTTCCTATCATG | 9,128 | S043, (traJ), traL, traE, traK, traB, traV, traA1, traA2 |
| 170-10f | CAGCTCGATCCGTTCTTCACCATTA | |||
| 5 | 170-9r | CGCACATTATCTCCAAGCACCGTTT | 9,003 | DsbC, traC |
| 170-9f | CAGCAACCCAAAGAAGAGCAAGTAC | |||
| 6 | AB571865-68599 | AAGGAAGTACGGTGGAAG | 6,975 | trhF, traW, traU, traN |
| AB571865-75573 | CAGGATCGTCAAAGTGAC | |||
| 7 | 170-5r | GCCGCAATGGATCGTTATCGTTTCT | 9,143 | |
| 170-5f | TGCTCTGCCGACAACATTGGTTTAC | |||
| 8 | 170-4r | ATTGGGTTGAAGGTTGGAAGGAGTG | 9,141 | |
| 170-4f | CGGCATACTCAGGTTCTCCTTCTTT | |||
| 9 | 170-3r | GGCTGGTTGTGGCGATATTGATGAT | 9,242 | |
| 170-3f | GCCTTCTGCCAAACCTAATACTCCT | |||
| 10 | 170-21r | GCCAAGTTCGGTAAGAGTGAGAGTT | 9,003 | ter |
| 170-21f | AGCGTTCTCAATGGTTGGGTTATCC | |||
| 11 | 170-20r | CCTCAAGATCCTTTCCCTATGGTTC | 9,142 | traF, traH, traG |
| 170-20f | CGACTGCCGTAATTTCTTGCTCTAG | |||
| 12 | 170-19r | CGGAAAGATACGAAGACGGATTACC | 9,390 | |
| 170-19f | CTCCAACTCCAATGACCTTCAAGAC |