Table 5.
Detection of traI and other antimicrobial resistance genes in donors and transconjugants by PCR.
| Relaxase* | Antimicrobial resistance genes | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Strain | traI (pAQU1) | traI (ICEVchMex) | tet(B) | tet(M) | blaCARB-9-like | mph(A)-like | mef(A)-like | sul2 | floR |
| 04Ya001 | + | − | + | + | − | − | − | − | − |
| TJ001 | + | − | + | + | − | − | − | − | ND |
| 04Ya007 | − | + | + | + | + | + | + | + | − |
| TJ007 | − | + | − | − | − | − | − | − | ND |
| 04Ya016 | + | − | + | + | − | − | − | − | − |
| TJ016 | + | − | + | + | − | − | − | − | ND |
| 04Ya090 | + | − | + | + | − | − | − | − | − |
| TJ090 | + | − | + | + | − | − | − | − | ND |
| 04Ya108 | − | − | + | + | + | + | + | + | − |
| TJ108 | − | − | + | + | + | + | + | + | ND |
| 04Ya265 | + | − | + | + | + | + | + | + | − |
| TJ265 | + | − | + | + | + | + | + | + | ND |
| 04Ya311 | + | − | + | + | + | + | + | + | + |
| TJW2 | + | − | + | + | + | + | + | + | + |
| W3110 | − | − | − | − | − | − | − | − | |
*
By the identification of the nucleotide sequence of amplicons obtained by PCR using MOBH1_traI062-1F and MOBH1_traI062-2R.