Figure 2.

Isometric tension (T_max in Eq. 2; left ordinate) and stiffness (S_max in Eq. 3; right ordinate) in 8Pi solution (A) and 0Pi solution (B) at saturating [Ca²⁺]. The 8Pi solution contained 8 mM Pi and its IS was 200 mM, whereas the 0Pi solution contained no added Pi and its IS was 150 mM. ^∗∗p < 0.01 and ^∗p < 0.05 compared to WT. The number of experiments (n) in A is 31 for WT, 38 for t/t, 32 for ADA, 31 for DAD, and 27 for SAS; in B, n = 14 for WT, t/t, ADA, and DAD, and n = 11 for SAS.