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Unbinding forces for C3d-Sbi-IV complexes. Steered MD simulations were performed for each complex, in which the center of mass of Sbi was pulled away from C3d at a rate of 10 Å/ns, and exerted force was reported every 20 fs. The area of the curves represents the mean ± SE of the force on Sbi in Complex 1 (dark gray) and Complex 2 (light gray) for five SMD trajectories.
