Figure 6.

Transformation of tubes into pearls in spreading cells. The cells expressing LimEΔ-GFP (green) were incubated for 30 min with latA in gently shaken suspension before they were transferred onto a glass surface and FM4-64 (red) was added. At that time no filamentous actin structures were detectable by the LimEΔ-GFP label. (A) A spreading cell protruding multiple tubes, the left one is subdivided into five pearls. (B) A tube protruded by a spreading cell, which is from top to bottom progressively organized into pearls. Numbers indicate seconds after the first frame in each panel. Bars, 10 μm. The pearling sequence shown in (B) is displayed in Movie S16.