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. 2014 Mar 4;106(5):1008–1019. doi: 10.1016/j.bpj.2013.12.043

Figure 2.

Figure 2

Acridine orange on the surface of a glass slide. Imaged emission intensity (pˆ + sˆ) (A) without and (B) with azimuthal scanning. pˆ/sˆ emission ratio (C) without and (D) with azimuthal scanning. A vertical and horizontal line scan is shown next to each image. Scale bar 5 μm.