Figure 4.

(A) DiO in a supported lipid bilayer was imaged with constant $\hat{\mathit{s}}$ polarization (left) and constant $\hat{\mathit{p}}$ polarization (right), whereas the excitation beam was fixed at $x_{TIRF}^{+}$ (no azimuthal scanning). (B) DiO imaged with azimuthal scanning with $\hat{\mathit{s}}$ polarization (left) and $\hat{\mathit{p}}$ polarization (right). (C) DiO imaged with $\hat{\mathit{s}}$ polarization (left) and $\hat{\mathit{p}}$ polarization (right) during galvanometer quivering around $x_{TIRF}^{+}$. Scale bar 1 μm.