Table 1.
Effects of the lipid bilayer composition on KcsA ligand gating
| Protein | Lipid | pH0.5 ± SD | nH ± SD | Rate ± SD |
|---|---|---|---|---|
| WT KcsA | DC18:1PC/POPG 3:1 | 5.00 ± 0.04 | 2.9 ± 0.2 | 203 ± 14 |
| WT KcsA | DC18:1PC/POPG 1:1 | 5.0 ± 0.2 | 3.2 ± 0.3 | 213 ± 49 |
| E71A KcsA | DC18:1PC/POPG 3:1 | 5.00 ± 0.04 | 3.3 ± 0.2 | 247 ± 5 |
| WT KcsAa | DC22:1PC/POPG 3:1 | 4.60 ± 0.05 | 2.1 ± 0.6 | 206 ± 34 |
| E71A KcsA | DC22:1PC/POPG 3:1 | 4.8 ± 0.1 | 2.4 ± 0.3 | 305 ± 9 |
| E71A KcsAb | DC22:1PC/POPG 3:1 | 4.2 ± 0.2 | 1.3 ± 0.2 | 344 ± 7 |
| E71A KcsAc | POPE/POPG 3:1 | 5.30 ± 0.01 | 4.4 ± 0.1 |
a
Values are given as the mean ± range/2, n = 2.
b
Single-mixing mode.
c
Data taken from Thompson et al. (28).