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B. thailandensis T3SS3 mutants activate NFκB. NFκB/293/GFP-Luc cells were infected with wildtype B. thailandensis (E264), B. thailandensis ∆bsaS mutant or stimulated with PMA and ionomycin for 6 hr (A) and 24 hr (B). Cells were lysed and assayed for luciferase activity.
