Table 2.
Plasmids used for cellular transfection in the study
| Plasmid | Relevant characteristic(s)a | Source or reference |
|---|---|---|
| pK18mobsacB |
Conjugative, suicide vector containing sacB gene, Kmr |
[42] |
| pUC18T-mini-Tn7T-Zeo-lox |
Source of zeocin resistance gene, Apr, Zeor |
[43] |
| pNFκB-SEAP |
Reporter vector containing NFκB enhancer element fused to SEAP gene, AmpR |
BD Clontech |
| pcDNA3.1/V5-His TOPO |
Expression Vector, AmpR |
Life Technologies |
| pCMV-FLAG-MAT-Tag-1 |
Expression Vector, AmpR |
Sigma |
| pcDNA-bopA |
bopA gene cloned into pcDNA3.1/V5-His TOPO by TA-cloning, AmpR |
This study |
| pCMV-bopC |
bopC gene cloned into pCMV-FLAG-MAT-Tag-1, AmpR |
This study |
| pRK5myc-BopE |
bopE gene cloned into pRK5myc, AmpR |
[23] |
| pRK5myc-SopE | sopE gene cloned into pRK5myc, AmpR | [23] |
a Abbreviations: AmpR, ampicillin resistant.