Table 3.

List of primers for this study

Primer name	Sequences (5′-3′)
Mutant construction
BsaM up for	AAGCTTCACGCGACGCGATTTTGAATTG
BsaM up rev	AAGCTTGCTCGCCGACGCAGAAAAATA
BsaM dn for	GAATTCAAGCTTGATCACGCGTCCTGGTATTT
BsaM dn rev	TTGGATCCAAGCGAGACGTAGATGCTG
BopA up for	CCAAGCTTGCATGCCTGCAGGTCTTGCTCTCGGTTGAAGG
BopA up rev	GAGGATCCCCGGGTATGCATCGACATTGATCATCC
BopA dn for	TACCCGGGGATCCTCGCATGAAGAACGCATGAAGA
BopA dn rev	CCATGATTACGAATTCGATTCTTGTTGCTCCGATGC
BopC up for	CCATGATTACGAATTCCCCGACCAGTTGAAGATGTC
BopC up rev	GAGGATCCCCGGGTAGAACCAATGCCTAGCCTCAC
BopC dn for	TACCCGGGGATCCTCCTGGGGTCGGTTTACATACG
BopC dn rev	CCAAGCTTGCATGCCTGCAGGAGCATCGCGAATACGAACT
BopE up for	CGGTACCCGGGGATCCAACAACCGCTCCTTCATCC
BopE up rev	TCATGTCTTGCTCTCGGTTG
BopE dn for	GAGAGCAAGACATGAGACGCTCGAAGCCACATAC
BopE dn rev	GGCCAGTGCCAAGCTTGTATTACGAGTCGGGGCTGA
Bt BsaM up for	GGCCAGTGCCAAGCTTTTTCCAGAAAAGCGAGCAAT
Bt BsaM up rev	GGCGATAAATGGCCTGATTA
Bt BsaM dn for	AGGCCATTTATCGCCGATCACGCGTCCTGGTATTT
Bt BsaM dn rev	CGGTACCCGGGGATCCAGCAGCGAGAAAGAAACGAA
Expression in mammalian cells
BopA for	GAGGAGTGGATGATCAATGTCG
BopA rev	GTTCTTCATGCTGTCTTCAGGC
BopC for	AAGCTTAAGATGCCGAGCATGACC
BopC rev	GGATCCTCATGCGAGTGGGGTGTC
Real-Time PCR
BopA RT for	TTCGTGCTGTTCGGGCTGA
BopA RT rev	TCGATACTTGAGCTCGCCTGACTT
BopC RT for	TACCGACGATCTCGTCAAAG
BopC RT rev	GCGTTCAAGGAAGTTAAGCC
BopE RT for	TCCTTCGCTTCGCTGAAGATCG [18]
BopE RT rev	ATTCGGCCGGCAAGTCTACG [18]
GAPDH for	CAATGACCCCTTCATTGACC [44]
GAPDH rev	GTTCACACCCATGACGAACATG [44]