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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Semin Oncol. 2014 Feb 7;41(2):259–266. doi: 10.1053/j.seminoncol.2014.02.001

Figure 5.

Figure 5

A Nodal High population of C8161 cells sorted using Nodal mRNA SmartFlares was plated on glass coverslips and fixed and permeabilized with methanol. The cells were then treated with a rabbit primary antibody to Nodal (Santa Cruz; H-110) and a mouse primary antibody to CD133 (Millipore; 17A6.1), followed by an Alexa dye 488-conjugated secondary antibody against the rabbit antibody (green; Nodal) and Alexa dye 594-conjugated secondary antibody against the mouse antibody (red; CD133). CD133 expression appears enriched in the Nodal High sorted subpopulation (A) and Western blot analysis of the Unsorted vs. the Nodal High subpopulations for CD133 protein expression shows an increase in the amount of CD133 glycosylated CD133 (130 kilodalton) protein in the Nodal High selected subpopulation vs. the Unsorted subpopulation (B; corrected for the amount of protein loaded per lane using β-Actin protein as a control, see Figure 3).