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. Author manuscript; available in PMC: 2014 May 20.
Published in final edited form as: Arthritis Rheum. 2013 Sep;65(9):2430–2440. doi: 10.1002/art.38041

Figure 1.

Figure 1

Ig VH–complementarity-determining region 3 (CDR3) amino acid sequences of singly sorted IgM+κ+CD27+ B cells from patients with hepatitis C virus–associated mixed cryoglobulinemia (HCV-MC). Ig VH and Ig Vκ reverse transcription–polymerase chain reaction was performed on the singly sorted B cells from 4 patients (LDU 125, 773, 1403, and 1432). Depicted are representative clones of each patient's predominant clonal heavy chain CDR3 (HCDR3) populations. Germline VH1-69 amino acid sequences of framework regions (FRs) and CDRs are shown, and sequences are listed by subject and B cell clone. Sequences are compared to those of germline VH1-69 sequences; identity is shown by dashed lines. The frequency of each clone among the total number of clones with identical HCDR3 is listed (at the far right). Complete nucleotide sequences are available from the GenBank database (accession nos. EF624068–EF624214 and JF944897).