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Anti-IgG1 activity of IgM positive for VH1-69/Vκ3-20 is dependent upon the presence of somatic hypermutation (SHM). Anti-IgG1 activity of wild-type, somatically hypermutated IgM (squares) and germline-reverted (REV) IgM (circles) is depicted for subject LDU 125 (A), subject 773 (B), subject 1403 (C), and subject 1432 (D). In A, mGO53 IgM is a negative control. Values are the mean ± SD.
