Fig. 3.

3D two-photon imaging of the entire vasculature of a transgenic Tg(kdrl:GFP) zebrafish head at 3 days post fertilization (dpf). The fish vasculature is labeled with GFP. (a-e) Selected x-y plane slices of two-photon Bessel light-sheet images. (f) x-z plane cross section of the 3D image stacks (g) y-z plane cross section of the 3D image stack. The full image set (512 × 512 × 577) can be viewed in Media 1 ^{(9.6MB, MP4)} . 3D images were acquired by moving the sample tube at a Nyquist sampling density of 1-μm per z step, with the imaging plane moving deeper into the fish as z increases (tube moves toward camera). The tube lens focal length was set to 70 mm, resulting in a lateral pixel size of 1.1 μm. The expose time was 1s per step. Scale bar: 100 μm.