Table 2.
Characteristics of primary antibodies used in immunohistochemical analysis.
| Protein | Number of cases | Clone | Company | Catalog number | Raised in | Positive controls | Antigen retrieval method | Dilution and incubation time for immunohistochemistry |
|---|---|---|---|---|---|---|---|---|
| IL-8 | 51† | polyclonal | Invitrogen Corporation, Camarillo, CA | AHC 0881 | rabbit | Normal tonsillar tissue | pH 6 (low) | 1 : 50, 18 h 4°C |
|
| ||||||||
| IL-6 | 51† | polyclonal | Santa Cruz Biotechnology, Santa Cruz, CA | SC 1265 | goat | Normal tonsillar tissue | pH 6 (low) | 1 : 50, 18 h 4°C |
|
| ||||||||
| SOCS-3 | 35† | polyclonal | Santa Cruz Biotechnology, Santa Cruz, CA | SC 9023 | rabbit | Cholangiocarcinoma | pH 6 (low) | 1 : 100, 18 h 4°C |
|
| ||||||||
| CXCR2 | 51† | monoclonal | R and D Systems, Abingdon, England | MAB 331 | mouse | Normal tonsillar tissue | pH 9 (high) | 1 : 100, 18 h 4°C |
|
| ||||||||
| VEGF [recognizes 165, 189, 206 aa isoforms of VEGF] |
41* | monoclonal | Pharmingen BD Company, San Diego, CA | clone G153-694 | mouse | Glioblastoma | pH 6 (low) | 1 : 40, 18 h 4°C |
|
| ||||||||
| p-STAT3 [specific at site Tyrosine 705] |
51† | monoclonal | Cell Signaling Technology Inc., Boston, MA, USA | D3A7 XP | rabbit | Human breast cancer | pH 6 (low) | 1 : 100, 18 h 4°C |
|
| ||||||||
| CD34 | 54† | monoclonal | Dako, Carpinteria, CA | clone QBEnd/10 | mouse | Kaposi sarcoma | pH 9 (high) | 1 : 50, 30 min room temperature |
*21 cases were excluded due to loss of antigen preservation.
†In the remaining cases, staining information was not available due to exhaustion of the available tissue.