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. 2014 May 5;2014:376191. doi: 10.1155/2014/376191

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Copyright © 2014 Akito Maeshima et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of follistatin on the production of extracellular matrix in kidneys after UUO. (a) Production of type I collagen (A–D), type III collagen (E–H), and fibronectin (I–L) in the UUO kidneys was examined by immunostaining. (A, E, I) normal kidney. (B, F, J) contralateral kidney. (C, G, K) saline-treated UUO kidney, 7 days. (D, H, L) follistatin-treated UUO kidney, 7 days. Type I collagen, type III collagen, and fibronectin (green). Magnification: ×100. (b) Quantitative analysis of extracellular matrix production. Type I collagen, type III collagen, and fibronectin-positive area in kidneys at 7 days after UUO was measured as described in Section 2. Values are mean ± SE (n = 5). *P < 0.05. N.S., not significant.