Figure 1.
Analysis of NDRG2 methylation in different grade gliomas and effects of NDRG2 methylation on patient survival. (A) Methylation frequency (%) shown in the bar plot; asterisk indicates significant differences between glioma grades (p<0.05). (B) Kaplan-Meier survival curves for survival (months) of all glioma patients stratified by NDRG2 methylation status; Log-rank test, χ2=5.99, df=1, p=0.014. (C) Representative MS-PCR for NDRG2 in different grade glioma tumor specimens. U represents amplification of unmethylated alleles, and M represents methylated alleles. Standard Bisulfite Converted Universal Methylated Human DNA (M contr.) and normal human peripheral lymphocytes (U contr.) served as positive and negative methylation controls, respectively. H2O - water control, GBM - glioblastoma (astrocytoma of WHO grade IV), AI, AII, and AIII designate astrocytomas of WHO grade I, II, and III, respectively.