Citrate Phosphate Buffer (22.2 mM Citric Acid, 55.6 mM Dibasic Na2HPO4, pH 5.4)

222 ml 0.1 M citric acid

278 ml 0.2 M dibasic Na2HPO4

500 ml MilliQ H2O

Mix citric acid, sodium phosphate, and 450 ml of MilliQ H2O. Adjust pH to 5.4 and q.s. to 1 L.

This solution is stable at room temperature.

Binding Reaction Buffer

Citrate Phosphate Buffer, pH 5.4 with

2% v/v 1 mM PefaBloc

1.5% w/v Octyl-β-D-glucopyranaside

Use right away.

1 mM PefaBloc

1 ml MilliQ H2O

25 mg PefaBloc SC (powder)

Make aliquots and store at -20 ˚C.

Borate Buffer Formula (12.5 mM Sodium tetraborate decahydrate, pH 8.2)

1.19 g sodium tetraborate decahydrate (Sigma)

250 ml MilliQ H2O

Dissolve sodium tetraborate decahydrate in 225 ml of MilliQ H2O. Adjust pH to 8.2 and q.s. to 250 ml.

This solution is stable at room temperature.

PBS-Tween (2.7 mM KCl, 1.5 mM KH2PO4, 136.9 mM NaCl, 8.9 mM Na2HPO4•7H2O, 0.05% Tween, pH 7.4)

4.5 L MilliQ H2O

500 ml 10x Dulbecco’s PBS (DPBS)

2.5 ml Tween 20

This solution is stable at room temperature.

Neutralization Buffer (50 mM Trizma HCl, pH 8.0)

475 ml MilliQ H2O

25 ml 1 M Trizma HCl

Mix and adjust pH to 8.0

This solution is stable at 4 °C.