Fig. 4.

One-step growth curves of wt PV type 1 Mahoney (PVM) and CRS2 at 33 and 37 °C in HeLa cells. HeLa R19 cells were infected with wt or CRS2 virus at an m.o.i. of 1. Cultures of infected cells were harvested a 0, 2, 4, 6, 8, 12 and 24 h post-infection (p.i.) at 33 °C (a) and 37 °C (b), respectively. Viral titres were determined by plaque assay (see Methods).