Table 1. List of the CRS mutants of 2CATPase, the corresponding amino acid and nucleotide changes, and their growth phenotypes.
| Mutants | Wild-type codon(s) | Alanine codon(s)* | Growth phenotype | Time of full CPE at indicated temperature† | ||
| 33 °C | 37 °C | 39.5 °C | ||||
| CRS1 (C269A) | TGT | gcT | Lethal | – | – | – |
| CRS2 (C272A/H273A) | TGTCAC | gcTgcC | ts | Tf | Tf | Passage 1 |
| CRS3 (C281A/C282A) | TGCTGT | gcCgcT | Lethal | – | – | – |
| CRS4 (C286A) | TGT | gcT | qi | Passage 1 | Passage 1 | Passage 1 |
*
Lower-case letters indicate the nucleotide changes.
†
–, No CPE, even after four blind passages; Tf, full CPE at transfection; passage 1, full CPE at passage 1.