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. Author manuscript; available in PMC: 2014 Nov 14.
Published in final edited form as: J Med Chem. 2013 Oct 28;56(21):8765–8780. doi: 10.1021/jm401232v

Figure 2.

Figure 2

(a) ATP-mediated excision of chain-terminating nucleotides by AZTr RT. Excision reactions were carried out as described in Materials and Methods. Assays were quenched at different times of reaction and monitored by gel electrophoresis. (b) Rate of nucleotide excision for AZT-terminated (●) and 21a-terminated (○) primers. (c). Fe2+- mediated site-specific footprinting assay. Reactions were carried out as described in Materials and Methods. The next incoming nucleotide (TTP) was added in increasing concentrations prior to the Fe2+-mediated cleavage. (d) Graphical representation of data shown in panel (c).