Figure 3.

(A) Gel filtration experiment (KW-803, Shodex) showing the complex formation of the MnmE dimer (blue) and the MnmG dimer (red), forming the α2β2 MnmEG complex (black). Twenty five microliters of 100-μM solutions were injected on the column. (B) The theoretical scattering curve (red) of the MnmEG docking model agrees well with the experimental scattering curve (blue). The residuals of the fits are also shown. (C) and (D): Docking model of the α2β2 MnmEG complex superposed on the ab initio shape as obtained by Dammif (gray).