Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Mar 31;42(9):6038–6051. doi: 10.1093/nar/gku232

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — SEC–MALS analysis of Vps75 suggests it forms a stable tetramer under physiological conditions. (A) SEC–MALS elution profiles of full-length Vps75 in 150 mM (blue) and 500 mM (red) sodium chloride. The molar mass over elution peaks is shown in corresponding colours. (B) As in (A) except Vps75 lacking the unstructured C-terminal domain was analysed (residues 1–223). The association of soluble Vps75 with Vps75 dimers immobilized to a probe via a biotin linkage was measured using bio-layer interferometry. Changes in the response units were measured in the presence of increasing concentrations of soluble Vps75 dimers (C). A fit to the binding response data was then used to calculate the dissociation constant, K_d (D).